Siberian Federal University

Issue

Journal of Siberian Federal University. Biology. 2023 16 (1)

Authors

Sibirtsev, Vladimir S.; Nechiporenko, Uliana Yu.; Kabanov, Vladimir L.; Kukin, Mikhail Yu.; Radin, Mikhail A.

Contact information

Sibirtsev, Vladimir S.: Saint Petersburg State Chemical and Pharmaceutical University Saint Petersburg, Russian Federation; ; ORCID: 0000-0003-0829-5213; Nechiporenko, Uliana Yu.: Saint Petersburg State Chemical and Pharmaceutical University Saint Petersburg, Russian Federation; ORCID: 0000-0002-4102-1129; Kabanov, Vladimir L.: All-Russia Research Institute for Food Additives Saint Petersburg, Russian Federation; ORCID: 0000-0001-9085-2984; Kukin, Mikhail Yu.: Scientific Research Institute of the Bakery Industry Saint Petersburg, Pushkin, Russian Federation; ORCID: 0000-0003-1722-4644; Radin, Mikhail A.: Saint Petersburg State Chemical and Pharmaceutical University Saint Petersburg, Russian Federation; ORCID: 0000-0001-9951-7955

Keywords

microbiological assay; antibiotic properties; plant extracts

Abstract

Recently, the content of biologically active substances (BAS) of natural origin in food, pharmaceutical, cosmetic, and other products manufactured and consumed by humans has been steadily decreasing. Among the most acceptable and common sources of such BAS are various plant extracts. Moreover, the problem of developing sufficiently objective and at the same time rapid and widely applicable methods for quantitative assessment of pro- and antibiotic properties of a large number of products, both new and already approved for use, is becoming increasingly urgent. Thus, the purpose of this study was 1) to develop a rapid and objective instrumental method for assessing pro- and antibiotic properties of various samples of food, pharmaceutical, cosmetic, and other products and 2) to analyze the influence of various plant extracts on the biochemical activity of typical representatives of human microbiota and pathogenic microflora by using this method. The bioassay procedure has been developed, which includes periodic (every 2 h) recording of changes in pH, redox potential, and electrical conductivity of a liquid nutrient test medium (NTM) inoculated with viable test microorganisms (TM) and incubated in the presence and absence of test samples (TS). This procedure was used to conduct comparative analysis of the pro- and antibiotic activity of different concentrations of subcritical whole extracts prepared from 10 different types of plant raw materials using liquefied CO2 against Escherichia coli, Lactobacillus acidophilus, and Staphylococcus aureus. The study shows that among the TS, the most active long-term antibiotic properties are exhibited by extracts of wormwood (Artemisia taurica) and wild rosemary (Ledum palustre) leaves and marsh calamus (Acorus calamus) roots at NTM concentrations of 3 vol.% or higher. The most active long-term probiotic properties were exhibited by extracts of common juniper (Juniperus communis) berries, elecampane (Inula helenium) roots, calamus (Acorus calamus) roots, and yarrow (Achilluea millefolium) leaves at NTM concentrations of 0.2 vol.%. The initial biological activity of most TS was greater than their long-term activity. At the same time, the mid-term (relative to the TS/TM interaction time) antibiotic activity of most TS was intermediate between their initial and long-term activity. Thus, it is obvious that the biological activity of food and other products containing various plant extracts is determined not only by the BAS composition of the product but also by the concentration of the extract in the product, the time of its interaction with living organisms (humans, their microbiota, etc.), and other factors. Moreover, the exact nature of these relationships in most cases can be established only empirically, by performing a considerable number of tests. These tests can be conveniently carried out using the procedure described in this work, which is less labor- and material-intensive than conventional microbiological methods. This procedure gives much more rapid, objective, and informative assessments of the effects of various samples of food, pharmaceutical, cosmetic, and other products and individual ingredients and additives on the dynamics of the vital activity of microorganisms (which, as shown in the work, reliably correlates with their biochemical activity)

Pages

109–124

EDN

ZZYKWQ

Paper at repository of SibFU

https://elib.sfu-kras.ru/handle/2311/150074

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).