Siberian Federal University

The sorption activity of octadecylsilica gel-based sorbent towards grosgemin has been investigated. Dynamic sorption curves in the concentration range of 0.5–2.5×10–3mg/mL grosgemin in acetonitrile solutions were plotted. The sorption characteristics: volume before “slip”, equilibrium volume and retention volume were determined graphically. On the basis of the obtained data the dynamic capacity of the sorbent was calculated. It was found that varying the concentration of acetonitrile (MeCN) affects the sorption characteristics of the sorbent in the dynamic regime: with increasing concentration, the “volume to slip” and the retention volume decrease significantly. Thus, the dynamic capacity decreases: from 1.39±0.02 at 10 % MeCN to 0.10±0.01mg/g at 50 % MeCN. Kinetic curves of sorption were constructed and it was shown that the maximum adsorption of grosgemin is reached 4 minutes after contact with octadecylsilica gel in the medium of 20 % acetonitrile solution. The mechanism of polymolecular adsorption of grosgemin according to the Brown-Emmett-Teller theory on the surface of octadecylsilica gel was determined. The values of sorption capacity from 0.0017 to 0.0252 mg/g obtained as a result of the work testify to the efficiency of using octadecylsilica gel for further solid- phase concentration of grosgemin as a stage of preparation of samples of extracts of plant raw materials of cornflower for chromatographic analysis